PCR/RT-PCR in situ light and electron microscopy

By Gérard Morel

Subjects: In situ, Electron Microscopy, Methods, Electron microscopy, Reação em cadeia por polimerase, Microscopy, Microscópio eletrônico (técnicas), Electron microscopes, Reverse transcriptase, DNA-Directed DNA Polymerase, Transcriptase inverse, Labortechnik, Dna, Technique, Polymerase Chain Reaction, Microscopie électronique, Microscopie, Polymerase-Kettenreaktion, Réaction en chaîne de la polymérase, Polymerase chain reaction

Description: "PCR/RT-PCR in situ Light and Electron Microscopy covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and RNA in tissues and cell cultures at the light and electron microscopic levels. Written by experts in this field, the book provides theoretical consideration, as well as practical approaches to in situ PCR. The authors provide detailed protocols for each step, including the preparation of tissue samples, the rationale for the design of primers, and revelation. They also emphasize the need for appropriate controls to meet the requirements of in situ PCR and RT-PCR specificity. Organized in a user-friendly two-column format, this book will provide you with tools necessary to perform and optimize these sensitive and powerful techniques in your research protocols."--BOOK JACKET.